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Active Recombinant E. coli eco Protein, His-tagged

Cat.No. : eco-794E
Product Overview : Recombinant E. coli eco (Accession # NP_416713) Ala21-Arg162, fused with a C-terminal 6-His tag, was produced in E. coli.
  • Specification
  • Gene Information
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Source : E. coli
Species : E. coli
Tag : His
Predicted N Terminal : Ala21
Form : Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 value is <1.0 nM, as measured under the described conditions.
Molecular Mass : Recombinant E. coli eco, His-tagged has a calculated MW of 17 kDa. In SDS-PAGE migrates as 18 kDa, reducing conditions.
Protein length : Ala21-Arg162
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 250 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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