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Recombinant Human AGRN Protein, His-tagged

Cat.No.: AGRN-875H
Product Overview: Recombinant Human AGRN (Accession # NP_940978) Ala1260-Pro2045, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO Cells
Species: Human
Tag: His
Predicted N Terminal: His
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells.The typical ED50 for this effect is 0.9 - 3.6 µg/mL after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass: Recombinant Human AGRN, His-tagged has a calculated MW of 83.5 kDa. In SDS-PAGE migrates as 100-110 kDa, reducing conditions.
Protein length: Ala1260-Pro2045
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: AGRN agrin [ Homo sapiens ]
Official Symbol: AGRN
Synonyms: AGRN; agrin; AGRIN; agrin proteoglycan;
Gene ID: 375790
mRNA Refseq: NM_198576
Protein Refseq: NP_940978
MIM: 103320
UniProt ID: O00468
Chromosome Location: 1p36.33

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