Active Recombinant Human AGRN Protein, His-tagged

• Cat.No.: AGRN-875H
• Product Overview: Recombinant Human AGRN (Accession # NP_940978) Ala1260-Pro2045, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells.The typical ED50 for this effect is 0.9 - 3.6 µg/mL after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human AGRN, His-tagged has a calculated MW of 83.5 kDa. In SDS-PAGE migrates as 100-110 kDa, reducing conditions.
• Protein length: Ala1260-Pro2045
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: AGRN agrin [ Homo sapiens ]
• Official Symbol: AGRN
• Synonyms: AGRN; agrin; AGRIN; agrin proteoglycan
• Gene ID: 375790
• mRNA Refseq: NM_198576
• Protein Refseq: NP_940978
• MIM: 103320
• UniProt ID: O00468
• Chromosome Location: 1p36.33