Active Recombinant Human AGRP

• Cat.No.: AGRP-890H
• Product Overview: Recombinant Human AGRP (Accession # O00253) Ser83-Thr132, fused with , was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Ser83
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to antagonize alpha -MSH-induced cAMP accumulation in HEK293 human embryonic kidney cells transfected with human Melanocortin-4 Receptor. Ollmann, M.M. et al. (1997) Science 278:135.The ED50 for this effect is typically 0.03-0.15 µg/mL in the presence of 10 ng/mL of alpha -MSH.
• Molecular Mass: Recombinant Human AGRP has a calculated MW of 5.6 kDa.
• Protein length: Ser83-Thr132
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: AGRP agouti related protein homolog (mouse) [ Homo sapiens ]
• Official Symbol: AGRP
• Synonyms: AGRP; agouti related protein homolog (mouse); agouti (mouse) related protein; agouti-related protein; Agrt; ART; ASIP2; AGRT
• Gene ID: 181
• mRNA Refseq: NM_001138
• Protein Refseq: NP_001129
• MIM: 602311
• UniProt ID: O00253
• Chromosome Location: 16q22