Active Recombinant Human CD5L Protein, His-tagged

• Cat.No.: CD5L-975H
• Product Overview: Recombinant Human CD5L (Accession # O43866) Ser20-Gly347, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Ser20-Gly347
• Predicted N Terminal: Ser20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit apoptosis on the J774A.1 mouse reticulum cell sarcoma macrophage cells. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.The presence of rhCD5L inhibited rhFas Ligand induced apoptosis by 70 - 90%. The ED50 for this effect is typically 1 - 4 µg/mL.
• Molecular Mass: Recombinant Human CD5L, His-tagged has a calculated MW of 36.9 kDa. In SDS-PAGE migrates as 43 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CD5L CD5 molecule-like [ Homo sapiens ]
• Official Symbol: CD5L
• Synonyms: CD5L; CD5 molecule-like; API6, apoptosis inhibitor 6 , CD5 antigen like (scavenger receptor cysteine rich family); CD5 antigen-like; Spalpha; CT-2; apoptosis inhibitor 6; igM-associated peptide; CD5 antigen-like (scavenger receptor cysteine rich family); AIM; API6; PRO229; SP-ALPHA
• Gene ID: 922
• mRNA Refseq: NM_005894
• Protein Refseq: NP_005885
• MIM: 602592
• UniProt ID: O43866
• Chromosome Location: 1q21-q23