"CRLF1&CLCF1" Related Products

Recombinant Human CRLF1&CLCF1

Cat.No.: CRLF1&CLCF1-1036H
Product Overview: Recombinant Human CRLF1&CLCF1 (Accession # O75462 (CLF-1) & Q9UBD9 (CLC)), fused with , was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO Cells
Species: Human
Predicted N Terminal: Ala38 (CLF-1) & Leu28 (CLC)
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells transfected with human CNTF R alpha.The ED50 for this effect is typically 0.5 - 3.5 ng/mL.
Molecular Mass: Recombinant Human CRLF1&CLCF1 has a calculated MW of 44.5 kDa (CLF-1) & 23 kDa (CLC). In SDS-PAGE migrates as 50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions.
Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: CRLF1 cytokine receptor-like factor 1 [ Homo sapiens (human) ]
Official Symbol: CRLF1
Synonyms: CLF; NR6; CISS; CISS1; CLF-1; zcytor5; cytokine receptor-like factor 1; cytokine receptor-like factor 1; class I cytokine receptor; cytokine type 1 receptor CRLP-1; cytokine-like factor 1
Gene ID: 9244
mRNA Refseq: NM_004750.4
Protein Refseq: NP_004741
MIM: 604237
UniProt ID: O75462
Chromosome Location: 19p12

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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