Recombinant Human CRLF1&CLCF1


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Recombinant Human CRLF1&CLCF1

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Cat.No. : CRLF1&CLCF1-1036H
Product Overview : Recombinant Human CRLF1&CLCF1 (Accession # O75462 (CLF-1) & Q9UBD9 (CLC)), fused with , was produced in Mouse myeloma cell line, NS0-derived.
Source : Mammalian cells
Species : Human
Predicted N Terminal : Ala38 (CLF-1) & Leu28 (CLC)
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured in a cell proliferation assay using TF-1 human erythroleukemic cells transfected with human CNTF R alpha.The ED50 for this effect is typically 0.5 - 3.5 ng/mL.
Molecular Mass : Recombinant Human CRLF1&CLCF1 has a calculated MW of 44.5 kDa (CLF-1) & 23 kDa (CLC). In SDS-PAGE migrates as 50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions.
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : CRLF1 cytokine receptor-like factor 1 [ Homo sapiens (human) ]
Official Symbol : CRLF1
Synonyms : CLF; NR6; CISS; CISS1; CLF-1; zcytor5; cytokine receptor-like factor 1; cytokine receptor-like factor 1; class I cytokine receptor; cytokine type 1 receptor CRLP-1; cytokine-like factor 1
Gene ID : 9244
mRNA Refseq : NM_004750.4
Protein Refseq : NP_004741
MIM : 604237
UniProt ID : O75462
Chromosome Location : 19p12

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