Active Recombinant Human CRLF1&CLCF1

• Cat.No.: CRLF1&CLCF1-1036H
• Product Overview: Recombinant Human CRLF1&CLCF1 (Accession # O75462 (CLF-1) & Q9UBD9 (CLC)), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Ala38 (CLF-1) & Leu28 (CLC)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells transfected with human CNTF R alpha.The ED50 for this effect is typically 0.5 - 3.5 ng/mL.
• Molecular Mass: Recombinant Human CRLF1&CLCF1 has a calculated MW of 44.5 kDa (CLF-1) & 23 kDa (CLC). In SDS-PAGE migrates as 50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CRLF1 cytokine receptor-like factor 1 [ Homo sapiens (human) ]
• Official Symbol: CRLF1
• Synonyms: CLF; NR6; CISS; CISS1; CLF-1; zcytor5; cytokine receptor-like factor 1; cytokine receptor-like factor 1; class I cytokine receptor; cytokine type 1 receptor CRLP-1; cytokine-like factor 1
• Gene ID: 9244
• mRNA Refseq: NM_004750.4
• Protein Refseq: NP_004741
• MIM: 604237
• UniProt ID: O75462
• Chromosome Location: 19p12