Recombinant Human DDAH1 Protein, Met & His-tagged DDAH1-1063H

Recombinant Human DDAH1 Protein, Met & His-tagged


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Recombinant Human DDAH1 Protein, Met & His-tagged

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Cat.No. : DDAH1-1063H
Product Overview : Recombinant Human DDAH1 (Accession # O94760) Ala2-Ser285, fused with an N-terminal Met and 6-His tag, was produced in E. coli.
Source : E. coli
Species : Human
Tag : & His
Predicted N Terminal : Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol and DTT.
Bio-activity : Measured by its ability to hydrolyze asymmetric dimethylarginine to L-citrulline and dimethylamine.The specific activity is >75 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human DDAH1, Met & His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 38 kDa, reducing conditions.
Protein length : Ala2-Ser285
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in 100 mM acetic acid containing at least 0.1% human or bovine serum albumin.
Gene Name : DDAH1 dimethylarginine dimethylaminohydrolase 1 [ Homo sapiens ]
Official Symbol : DDAH1
Synonyms : DDAH1; dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH; DDAHI; DDAH-1; dimethylargininase-1; NG, NG-dimethylarginine dimethylaminohydrolase; FLJ21264; FLJ25539;
Gene ID : 23576
mRNA Refseq : NM_001134445
Protein Refseq : NP_001127917
MIM : 604743
UniProt ID : O94760
Chromosome Location : 1p22

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