||Recombinant Human USP1&WDR48 (Accession # O94782 (USP1), Q8TAF3 (UAF1)), fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
||Baculovirus Sf21 Insect Cells
||X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
||Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 µM.
||Recombinant Human USP1&WDR48, His-tagged has a calculated MW of 89 kDa (USP1), 77 kDa (UAF1).
||>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).