Recombinant Human CD19 Protein, His tagged, Biotinylated

• Cat.No.: CD19-558H
• Product Overview: The recombinant human CD19 ECD is expressed as a 271 amino acid protein consisting of Pro20 - Val279 region of CD19 (UniProt accession #P15391) and a C-terminal His-tag. It contains 5 potential sites for N-linked glycosylation. Calculated PI: 7.03 Calculated extinction coefficients (M-1 cm-1, at 280nm): 61355

Specification

• Species: Human
• Source: Human cells
• Tag: His
• Protein Length: 20-279 aa
• Conjugation/Label: Biotin
• Form: Supplied at 0.5 mg/mL in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
• Bio-activity: Binds anti-CD19 monoclonal antibody human IgG1 with high affinity (KD < 1 nM - see the Technical Data) measured by ELISA with immobilized CD19 ECD protein (100 ng/well).
• Molecular Mass: 29.9 kDa
• AASequence: PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVSTGHHHHHHHH
• Endotoxin: < 0.1 EU/μg by LAL
• Purity: >95% judged by SDS-PAGE under reducing condition

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens (human) ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391