Recombinant Human ERCC5 protein, MYC/DDK-tagged

• Cat.No.: ERCC5-6563H
• Product Overview: Recombinant Human ERCC5, fussed with MYC/DDK tag at C-terminal, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV-induced damage. The protein may also function in other cellular processes, including RNA polymerase II transcription, and transcription-coupled DNA repair. Mutations in this gene cause xeroderma pigmentosum complementation group G (XP-G), which is also referred to as xeroderma pigmentosum VII (XP7), a skin disorder characterized by hypersensitivity to UV light and increased susceptibility for skin cancer development following UV exposure. Some patients also develop Cockayne syndrome, which is characterized by severe growth defects, mental retardation, and cachexia. Read-through transcription exists between this gene and the neighboring upstream BIVM (basic, immunoglobulin-like variable motif containing) gene.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 133.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: ERCC5 excision repair cross-complementing rodent repair deficiency, complementation group 5 [ Homo sapiens ]
• Official Symbol: ERCC5
• Synonyms: ERCC5; excision repair cross-complementing rodent repair deficiency, complementation group 5; ERCM2, xeroderma pigmentosum, complementation group G , XPGC; DNA repair protein complementing XP-G cells; Cockayne syndrome; XPG-complementing protein; DNA excision repair protein ERCC-5; xeroderma pigmentosum, complementation group G; XPG; UVDR; XPGC; COFS3; ERCM2
• Gene ID: 2073
• mRNA Refseq: NM_000123
• Protein Refseq: NP_000114
• MIM: 133530
• UniProt ID: P28715
• Chromosome Location: 13q22-q34
• Pathway: DNA Repair, organism-specific biosystem; Dual incision reaction in GG-NER, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Formation of incision complex in GG-NER, organism-specific biosystem; Formation of transcription-coupled NER (TC-NER) repair complex, organism-specific biosystem; Global Genomic NER (GG-NER), organism-specific biosystem; Nucleotide Excision Repair, organism-specific biosystem
• Function: DNA binding; bubble DNA binding; double-stranded DNA binding; endodeoxyribonuclease activity; endonuclease activity; endonuclease activity; metal ion binding; protein N-terminus binding; protein binding; protein homodimerization activity; single-stranded DNA binding; single-stranded DNA binding