Recombinant Full Length Human ERCC5 Protein, C-Flag-tagged

• Cat.No.: ERCC5-1392HFL
• Product Overview: Recombinant Full Length Human ERCC5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV-induced damage. The protein may also function in other cellular processes, including RNA polymerase II transcription, and transcription-coupled DNA repair. Mutations in this gene cause xeroderma pigmentosum complementation group G (XP-G), which is also referred to as xeroderma pigmentosum VII (XP7), a skin disorder characterized by hypersensitivity to UV light and increased susceptibility for skin cancer development following UV exposure. Some patients also develop Cockayne syndrome, which is characterized by severe growth defects, cognitive disability, and cachexia. Read-through transcription exists between this gene and the neighboring upstream BIVM (basic, immunoglobulin-like variable motif containing) gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 133.1 kDa
• AA Sequence: MGVQGLWKLLECSGRQVSPEALEGK ILAVDISIWLNQALKGVRDRHGNSI ENPHLLTLFHRLCKLLFFRI RPIFVFDGDAPLLKKQTLVKRRQRK DLASSDSRKTTEKLLKTFLKRQAIK TAFRSKRDEALPSLTQVRRE NDLYVLPPLQEEEKHSSEEEDEKEW QERMNQKQALQEEFFHNPQAIDIES EDFSSLPPEVKHEILTDMKE FTKRRRTLFEAMPEESDDFSQYQLK GLLKKNYLNQHIEHVQKEMNQQHSG HIRRQYEDEGGFLKEVESRR VVSEDTSHYILIKGIQAKTVAEVDS ESLPSSSKMHGMSFDVKSSPCEKLK TEKEPDATPPSPRTLLAMQA ALLGSSSEEELESENRRQARGRNAP AAVDEGSISPRTLSAIKRALDDDED VKVCAGDDVQTGGPGAEEMR INSSTENSDEGLKVRDGKGIPFTAT LASSSVNSAEEHVASTNEGREPTDS VPKEQMSLVHVGTEAFPISD ESMIKDRKDRLPLESAVVRHSDAPG LPNGRELTPASPTCTNSVSKNETHA EVLEQQNELCPYESKFDSSL LSSDDETKCKPNSASEVIGPVSLQE TSSIVSVPSEAVDNVENVVSFNAKE HENFLETIQEQQTTESAGQD LISIPKAVEPMEIDSEESESDGSFI EVQSVISDEELQAEFPETSKPPSEQ GEEELVGTREGEAPAESESL LRDNSERDDVDGEPQEAEKDAEDSL HEWQDINLEELETLESNLLAQQNSL KAQKQQQERIAATVTGQMFL ESQELLRLFGIPYIQAPMEAEAQCA ILDLTDQTSGTITDDSDIWLFGARH VYRNFFNKNKFVEYYQYVDF HNQLGLDRNKLINLAYLLGSDYTEG IPTVGCVTAMEILNEFPGHGLEPLL KFSEWWHEAQKNPKIRPNPH DTKVKKKLRTLQLTPGFPNPAVAEA YLKPVVDDSKGSFLWGKPDLDKIRE FCQRYFGWNRTKTDESLFPV LKQLDAQQTQLRIDSFFRLAQQEKE DAKRIKSQRLNRAVTCMLRKEKEAA ASEIEAVSVAMEKEFELLDK AKRKTQKRGITNTLEESSSLKRKRL SDSKRKNTCGGFLGETCLSESSDGS SSEDAESSSLMNVQRRTAAK EPKTSASDSQNSVKEAPVKNGGATT SSSSDSDDDGGKEKMVLVTARSVFG KKRRKLRRARGRKRKTTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Nucleotide excision repair

Gene Information

• Gene Name: ERCC5 ERCC excision repair 5, endonuclease [ Homo sapiens (human) ]
• Official Symbol: ERCC5
• Synonyms: XPG; UVDR; XPGC; COFS3; ERCM2; ERCC5-201
• Gene ID: 2073
• mRNA Refseq: NM_000123.4
• Protein Refseq: NP_000114.3
• MIM: 133530
• UniProt ID: P28715