Recombinant Human SIGLEC9 protein, Fc-tagged SIGLEC9-677H

Recombinant Human SIGLEC9 protein, Fc-tagged

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Recombinant Human SIGLEC9 protein, Fc-tagged

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Cat.No. : SIGLEC9-677H
Product Overview : Recombinant Human SIGLEC9 (Gln18-Gly348) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : siglec-9 (HGMW-approved symbol SIGLEC9) a member of the sialic acid-binding Ig-like lectin (Siglec) family, which belongs to the immunoglobulin superfamily (IgSF). SIGLEC9 shows a high degree of homology to many members of the siglec family, including siglec-7 (80%), siglec-8 (72%), siglec-5 (65%), and CD33 (64%). This high degree of homology is also conserved in the extracellular Ig-like domains. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains。Siglec-9 with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Gln18
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 62.5 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln18-Gly348
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of SIGLEC9-677H :

Human Siglec-9, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of SIGLEC9-677H :

Immobilized Human Siglec-9, Fc Tag at 10 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.156-2.5 μg/mL (QC tested).

Bioactivity-BLI :

Loaded Human Siglec-9, Fc Tag on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0.85 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Gene Name : SIGLEC9
Official Symbol : SIGLEC9
Synonyms : SIGLEC9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; CD329; protein FOAP-9; CDw329; FOAP-9; siglec-9; OBBP-LIKE
Gene ID : 27180
mRNA Refseq : NM_001198558
Protein Refseq : NP_001185487
MIM : 605640
UniProt ID : Q9Y336

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