||Native Human APOA1 was purified from Human Plasma HDL.
||Apolipoprotein AI promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein AI is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77aa; an 18aa signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein AI is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein AI gene are associated with HDL deficiency and Tangier disease.
||Human Plasma HDL
||Supplied as a liquid in 20mM Tris-HCl, pH 7.4, 0.15M sodium chloride, 0.5mM EDTA, 0.02% sodium azide.
||ELISA (EL/EIA). Suitable for use in ELISA. Other applications not tested.
||Small volumes of Apolipoprotein A-I lipoprotein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
||May be stored at 4 centigrade for short-term only. For long-term storage, aliquot and store at -20 centigrade. Aliquots are stable for at least 6 months at -20 centigrade. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.