Recombinant Human HtrA Serine Peptidase 2, His-tagged HTRA2-56H

Recombinant Human HtrA Serine Peptidase 2, His-tagged

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Recombinant Human HtrA Serine Peptidase 2, His-tagged

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Cat. No. : HTRA2-56H
Product Overview : Recombinant Human HtrA2 amino acids 134-458 His-Tag fusion protein produced inE.Coliis a single, non-glycosylated polypeptide chain having a molecular mass of 32 kDa. The HtrA2 is purified by proprietary chromatographic techniques.
Description : HtrA2 also called Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.
Source : Escherichia Coli.
Amino Acid Sequence : MAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGF VVA ADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT L PLGR SADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EY IQ TD AAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRG EK KNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHK VILGSPA HRA GLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGR ETLTLYV TP EVTEGSHH HHHH.
Physical Appearance : Sterile filtered clorless solution.
Purity : Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 50 mM NaCl, 1 mM DTT, and 20% Glycerol.
Stability : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
PDB rendering based on 1lcy. :

Gene Name : HTRA2 HtrA serine peptidase 2 [ Homo sapiens ]
Synonyms : HTRA2 ; HtrA serine peptidase 2; OMI; PARK13; PRSS25; protease, serine, 25;Serine protease HTRA2, mitochondrial;High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine proteinase OMI; Serine protease 25; EC 3.4.21.108; HtrA-like serine protease
Gene ID : 27429
mRNA Refseq : NM_013247
Protein Refseq : NP_037379
MIM : 606441
UniProt ID : O43464
Chromosome Location : 2p12
Pathway : Parkinson"s disease
Function : peptidase activity; serine-type endopeptidase activity; unfolded protein binding

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