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Recombinant Human Nicotinamide N-methyltransferase, His-tagged

Cat. No.: NNMT-143H
Product Overview: Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 31.7 kDa (284aa).
Description: NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Source: Escherichia Coli.
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol.
Purity: > 95% by SDS - PAGE.
Concentration: 1 mg/ml (determined by Bradford assay).
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDYLEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQLL SAC E SFKE IVVTD YSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCVLSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNEGLFSLVARKL SRPL
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
Synonyms: NNMT; nicotinamide N-methyltransferase; EC 2.1.1.1.
Gene ID: 4837
mRNA Refseq: NM_006169
Protein Refseq: NP_006160
MIM: 600008
UniProt ID: P40261
Chromosome Location: 11q23.1
Pathway: Nicotinate and nicotinamide metabolism ; Biological oxidations
Function: methyltransferase activity; nicotinamide N-methyltransferase activity; transferase activity

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