Recombinant Rat Rap1a

• Cat.No.: Rap1a-255R
• Product Overview: Recombinant Rat RAP produced inE.Coliis a single, non-glycosylated polypeptide chain. The RAP has a molecular mass of 38,862 Dalton. The RAP is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Rap1a-255R
• Description: Trace amine-associated receptor 1 is a protein that in humans is encoded by the TAAR1 gene. TAAR1 is a G protein-coupled receptor activated by trace amines. Trace amines are endogenous amine compounds that account for less than 1% of the biogenic amines in most brain regions.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: The protein (1mg/ml) was lyophilized after from a sterile solution containing TBS pH-7.5, 0.1% BSA and 0.09% NaN3.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Dimers And Aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rrRAP.
• Solubility: It is recommended to reconstitute the lyophilized rrRAP in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized rrRAP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rrRAP should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Rap1a RAP1A, member of RAS oncogene family [ Rattus norvegicus ]
• Synonyms: Rap1a; RAP1A, member of RAS oncogene family; RAP-1A; rap 1A; MGC93785; MGC94873; Ras-related protein RAP-1A; RAS-related protein 1a
• Gene ID: 295347
• mRNA Refseq: NM_001005765
• Protein Refseq: NP_001005765
• UniProt ID: P62836
• Chromosome Location: 2q34
• Pathway: Chemokine signaling pathway; Focal adhesion; Leukocyte transendothelial migration; Long-term potentiation; MAPK signaling pathway; Neurotrophin signaling pathway; Renal cell carcinoma
• Function: GTP binding; GTPase activity; Ras GTPase binding; nucleotide binding; protein binding