||Recombinant human proliferating cell nuclear antigen produced inSF9is a glycosylated, polypeptide chain. The recombinant human proliferating cell nuclear antigen has a molecular mass of 29,794 Dalton. The recombinant human proliferating cell nuclear antigen is purified by proprietary chromatographic techniques.
||Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis. Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10% of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.
||Sf9 insect cells.
||Recombinant Human PCNA is supplied at a of 1mg/ml in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.
||1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.
||Greater than 90% (SDS-PAGE).
||Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
||0.5-1.1µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.