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Recombinant Human Proliferating Cell Nuclear Antigen

Cat.No. : PCNA-174H
Product Overview : Recombinant human proliferating cell nuclear antigen produced inSF9is a glycosylated, polypeptide chain. The recombinant human proliferating cell nuclear antigen has a molecular mass of 29,794 Dalton. The recombinant human proliferating cell nuclear antigen is purified by proprietary chromatographic techniques.
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Cat. No. : PCNA-174H
Description : Proliferating cell nuclear antigen (PCNA), a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis. Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10% of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.
Source : Sf9 insect cells.
Formulation : Recombinant Human PCNA is supplied at a of 1mg/ml in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.
Immunological Functions : 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.
Purity : Greater than 90% (SDS-PAGE).
Applications : Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating Concentration : 0.5-1.1µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage Conditions : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Gene Name : PCNA proliferating cell nuclear antigen [ Homo sapiens ]
Synonyms : MGC8367; PCNA;Proliferating cell nuclear antigen; Cyclin;proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
Gene ID : 5111
mRNA Refseq : NM_002592
Protein Refseq : NP_002583
MIM : 176740
UniProt ID : P12004
Chromosome Location : 20pter-p12
Pathway : Base excision repair; Cell cycle; DNA replication; Mismatch repair; Nucleotide excision repair; Cell Cycle, Mitotic; DNA Repair; DNA Replication; Telomere Maintenance
Function : DNA binding; DNA polymerase processivity factor activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can PCNA levels in tumor tissues provide prognostic information for cancer patients? 05/18/2021

Yes, elevated PCNA levels in tumor tissues are associated with a poorer prognosis in many cancer types.

Are there any genetic mutations associated with PCNA that impact clinical outcomes? 04/12/2021

Mutations in the PCNA gene can lead to DNA repair deficiencies and may be associated with increased cancer risk and poor clinical outcomes.

How is PCNA protein relevant in cancer diagnosis and treatment? 03/01/2020

PCNA is often overexpressed in cancer cells, making it a potential diagnostic marker and a target for cancer therapies.

Are there any PCNA-targeted therapies currently in clinical trials or use? 03/02/2018

Yes, some clinical trials are exploring drugs that target PCNA, primarily in the context of cancer treatment.

How is PCNA protein involved in DNA repair pathways, and how is this relevant to clinical applications 01/15/2016

PCNA acts as a scaffold for various DNA repair proteins, making it crucial for maintaining genomic stability and relevant in DNA repair-based therapies.

Customer Reviews (3)

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Reviews
01/04/2023

    In protein electron microscopy structure analysis, PCNA protein proved to be an invaluable tool, allowing for the precise determination of protein structures with remarkable clarity and resolution.

    06/17/2017

      In my ELISA experiments, PCNA protein consistently provided reliable and reproducible results, with high sensitivity and specificity.

      04/07/2016

        I enthusiastically endorse PCNA protein as it delivers exceptional performance in a wide range of applications.

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