Human PCNA Knockdown Cell Lysate

• Cat.No.: PCNA-265HKCL
• Product Overview: WB-validated PCNA Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 29 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg PCNA KD HeLa cell lysate
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: PCNA proliferating cell nuclear antigen [ Homo sapiens (human) ]
• Official Symbol: PCNA
• Synonyms: PCNA; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein; MGC8367
• Gene ID: 5111
• mRNA Refseq: NM_002592
• Protein Refseq: NP_002583
• MIM: 176740
• UniProt ID: P12004