Recombinant B.subtilis Copper Transporter ATPase, Domain A

• Cat.No.: copA-440B
• Product Overview: Recombinant B.subitilis CopA Domain A (Copper-transporting P-type ATPase copA, CopA protein) cloned from B.subitilis cDNA with a C-terminal purification tag was expressed inE.coli. It consists of the soluble domain A of CopA from B.subtilis (residues 1-71, swissprot accession O32220). The protein is mutated in the S46V position. The C-terminal tag is cleaved during purification. MW = 8.1 kDa.

Specification

• Cat. No.: copA -440S
• Source: Escherichia Coli.
• Purity: 95% by SDS-PAGE.The protein was observed as a single band migrating at a molecular weight of <10 kDa.
• Supplied As: 1.0mg/ml in 20mM potassium phosphate buffer pH7.0, DTT 2mM (Dithiothreitol). The concentration is calculated from the absorbance at 280nm (e280 = 2680 M^-1cm^-1).
• Characteristics: Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 2mM.
• Storage: -20ºC. The protein is stable at 4ºC for at least 2 weeks and at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.

Gene Information

• Gene Name: copA copper transporter ATPase [Bacillus subtilis subsp. subtilis str. 168]
• Synonyms: copA; copper transporter ATPase
• Gene ID: 937985
• Protein Refseq: NP_391230