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Recombinant B.subtilis Copper Transporter ATPase, Domain B

Cat. No.: copA -441B
Product Overview: Recombinant B.subitilis CopA Domain B (Copper-transporting P-type ATPase copA, CopA protein) cloned from B.subitilis cDNA with a C-terminal purification tag was expressed in E. coli. It consists of the soluble domain B of CopA from B.subtilis (residues 73-147, swissprot accession O32220). The C-terminal tag is cleaved during purification. MW = 8.8 kDa.
Source: Escherichia Coli.
Purity: 95% by SDS-PAGE.The protein was observed as a single band migrating at a molecular weight of <10 kDa.
Supplied As: 1.0mg/ml in 20mM potassium phosphate buffer pH7.0, DTT 2mM (Dithiothreitol). The concentration is calculated from the absorbance at 280nm (e280 = 2680 M-1 cm-1).
Characteristics: Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 2mM.
Storage: -20ºC. The protein is stable at 4ºC for at least 2 weeks and at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.
Gene Name: copA copper transporter ATPase [Bacillus subtilis subsp. subtilis str. 168]
Synonyms: copA; copper transporter ATPase
Gene ID: 937985
Protein Refseq: NP_391230

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