||Recombinant S.meliloti Cox11 (Cytochrome C oxidase assembly protein) cloned from S.meliloti Cdna was expressed inE.coli.It consists of the soluble domain of the Cox11, it lacks the transmembrane segment (residues 36-198, swissprot accession Q92RG6). MW = 18.2 kDa.
||> 95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight of (< 20 kDa).
||1.0 mg/ml in 20mM KH2PO4/K2HPO4 pH7.0, 2mM DTT (Dithiothreitol), 0.5mM EDTA. The concentration is calculated from the absorbance at 280nm (e280 = 13490 M-1cm-1).
||Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 2mM.
||-20ºC. The protein is stable at 4ºC for at least 2 weeks and at 25ºC for at least several hours. After initial defrost, aliquot protein into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.