Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human PDGFB

Cat.No. : PDGFB-146H
Product Overview : Recombinant Human platelet-derived growth factor was expressed in modifiedhuman 293 cells.
  • Specification
  • Gene Information
  • Related Products
Cat. No. : PDGFB-146H
Description : The platelet-derived growth factor (PDGF) family consists of four different polypeptide chains; PDGF-A, PDGF-B, PDGF-C and PDGF-D, sharing approximately 25% sequence homology. The biological effects of PDGF-B are exerted via two receptor tyrosine kinases, PDGFRA and PDGFRB. PDGF-B is expressed predominantly in platelets and the heart, but has been detected in the majority of human organs. PDGF-B is a potent mitogen for mesenchymal cells and plays a critical role in wound healing and tumor development. PDGF-B is an important growth factor contributing to angiogenesis and possibly lymphatic metastasis due to lymphangiogenesis. Additionally, PDGF-B plays a crucial role in wound healing. Studies have also suggested that PDGF-B is involved in the CNS including having a neuroprotective or neurotrophic effect in the developing or adult CNS, promoting angiogenic responses to injury in the CNS and remyelination..
Source : human 293 cells.
Molecular Mass : Under reducing conditions PDGF-B migrates as a broad band between 15.2 and 16.1 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified PDGF-B polypeptide that has a predicted monomeric molecular mass of 12.29 kDa.
PI : PDGF-B has a predicted pI of 9.38.
% Carbohydrate : purified PDGF-B consists of 30-45% carbohydrate by weight.
Glycosylation : PDGF-B contains N-linked oligosaccharides and a predicted O-linked Mannose.
Purity : >95%, as determined by SDS-PAGE, visualized by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C.
Activity : The ED50of PDGF-B is typically 14-25 ng/mL as measured in a cell proliferation assay using the murine Balb/3T3 fibroblast cell line.
Theoretical Sequence : SLGSLTI AEPAMIAECK TRTEVFEISRRLI DRTNANFLVWPPC VEVQRCSGCCNNR NV QCRP TQVQLRPV QVRKI EIVRK KPIFKK ATVTLEDHLACKCETVAAARPV.
Gene Name : PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ]
Synonyms : platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; platelet-derived growth factor beta; becaplermin; PDGF, B chain; platelet-derived growth factor 2; platelet-derived growth factor, B chain;Platelet-derived growth factor, beta polypeptide (oncogene SIS); oncogene SIS; Becaplermin; PDGF-2; PDGF subunit B; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor B chain
Gene ID : 5155
mRNA Refseq : NM_002608
Protein Refseq : NP_002599
MIM : 190040
UniProt ID : P01127
Chromosome Location : 22q13.1
Pathway : Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Hemostasis; Signaling by PDGF
Function : cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
In what capacity does PDGFB contribute to the intricate processes of angiogenesis and vascular remodeling? 07/26/2021

By potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.

How does PDGFB intricately contribute to the development and advancement of certain malignancies? 02/08/2020

PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.

In what ways is PDGFB implicated in the pathogenesis and progression of fibrotic disorders? 12/16/2018

PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.

What novel therapeutic avenues are being explored targeting the PDGFB/PDGFRβ signaling axis for interventional strategies? 06/28/2018

Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.

How does PDGFB actively participate in the intricate mechanisms governing wound healing and tissue regeneration? 10/22/2017

PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.

What therapeutic strategies target PDGFB for disease treatment? 07/21/2017

Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.

How does PDGFB orchestrate cellular proliferation and growth in diverse tissues and organs? 03/07/2016

PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.

Customer Reviews (3)

Write a review
Reviews
12/27/2018

    The seal of the reagent tubes is excellent, preventing evaporation and leakage.

    05/08/2018

      With its unique design and construction, the experimental reagent provides me with a fresh experimental experience.

      03/21/2016

        The stability of this reagent is exceptional, ensuring the reliability of the experiment.

        Ask a Question for All PDGFB Products

        Required fields are marked with *

        My Review for All PDGFB Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends