Active Recombinant Human PDGFB
Cat.No. : | PDGFB-146H |
Product Overview : | Recombinant Human platelet-derived growth factor was expressed in modifiedhuman 293 cells. |
- Specification
- Gene Information
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Cat. No. : | PDGFB-146H |
Description : | The platelet-derived growth factor (PDGF) family consists of four different polypeptide chains; PDGF-A, PDGF-B, PDGF-C and PDGF-D, sharing approximately 25% sequence homology. The biological effects of PDGF-B are exerted via two receptor tyrosine kinases, PDGFRA and PDGFRB. PDGF-B is expressed predominantly in platelets and the heart, but has been detected in the majority of human organs. PDGF-B is a potent mitogen for mesenchymal cells and plays a critical role in wound healing and tumor development. PDGF-B is an important growth factor contributing to angiogenesis and possibly lymphatic metastasis due to lymphangiogenesis. Additionally, PDGF-B plays a crucial role in wound healing. Studies have also suggested that PDGF-B is involved in the CNS including having a neuroprotective or neurotrophic effect in the developing or adult CNS, promoting angiogenic responses to injury in the CNS and remyelination.. |
Source : | human 293 cells. |
Molecular Mass : | Under reducing conditions PDGF-B migrates as a broad band between 15.2 and 16.1 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified PDGF-B polypeptide that has a predicted monomeric molecular mass of 12.29 kDa. |
PI : | PDGF-B has a predicted pI of 9.38. |
% Carbohydrate : | purified PDGF-B consists of 30-45% carbohydrate by weight. |
Glycosylation : | PDGF-B contains N-linked oligosaccharides and a predicted O-linked Mannose. |
Purity : | >95%, as determined by SDS-PAGE, visualized by Coomassie Brilliant Blue. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. |
Activity : | The ED50of PDGF-B is typically 14-25 ng/mL as measured in a cell proliferation assay using the murine Balb/3T3 fibroblast cell line. |
Theoretical Sequence : | SLGSLTI AEPAMIAECK TRTEVFEISRRLI DRTNANFLVWPPC VEVQRCSGCCNNR NV QCRP TQVQLRPV QVRKI EIVRK KPIFKK ATVTLEDHLACKCETVAAARPV. |
Gene Name : | PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ] |
Synonyms : | platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; platelet-derived growth factor beta; becaplermin; PDGF, B chain; platelet-derived growth factor 2; platelet-derived growth factor, B chain;Platelet-derived growth factor, beta polypeptide (oncogene SIS); oncogene SIS; Becaplermin; PDGF-2; PDGF subunit B; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor B chain |
Gene ID : | 5155 |
mRNA Refseq : | NM_002608 |
Protein Refseq : | NP_002599 |
MIM : | 190040 |
UniProt ID : | P01127 |
Chromosome Location : | 22q13.1 |
Pathway : | Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Hemostasis; Signaling by PDGF |
Function : | cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionBy potently stimulating the migratory and proliferative capabilities of smooth muscle cells and pericytes, PDGFB plays a pivotal role in regulating angiogenesis and orchestrating vascular remodeling events, thereby ensuring proper tissue perfusion and function.
PDGFB signaling assumes a critical role in fueling tumor growth, angiogenesis, and metastasis. PDGFB, acting through its activation of PDGFRβ, elicits a pro-tumorigenic cascade that promotes tumor cell proliferation, survival, migration, and invasion, while concurrently fueling tumor vascularization and distant metastatic spread.
PDGFB emerges as a significant contributor to the pathogenesis of fibrotic diseases by prompting fibroblast activation and proliferation. These dysregulated processes lead to excessive extracellular matrix deposition and subsequent tissue scarring. Furthermore, PDGFB exacerbates fibrosis through induction of myofibroblast differentiation.
Promisingly, preclinical and clinical studies have investigated diverse approaches targeting the PDGFB/PDGFRβ signaling axis for therapeutic intervention in fibrotic disorders and certain neoplastic conditions. Inhibitors directed against PDGFB or PDGFRβ have exhibited considerable potential, effectively blocking downstream signaling cascades activated by PDGFB, thereby hampering cell proliferation and angiogenesis.
PDGFB, released by platelets and macrophages at inflammatory sites, acts as a key modulator in recruiting and activating fibroblasts. This activation not only promotes collagen synthesis but also initiates tissue remodeling, thus expediting the wound healing cascade and tissue integrity restoration.
Several therapeutic strategies aim to target PDGFB for disease treatment. Inhibitors of PDGFB, such as tyrosine kinase inhibitors, have been developed and used in clinical settings. These inhibitors can selectively block the activation of PDGF receptors and inhibit downstream signaling pathways, thereby suppressing excessive cell proliferation and migration. Additionally, approaches targeting PDGFB expression, such as gene therapy or RNA interference, hold promise as potential treatments for diseases associated with PDGFB dysregulation.
PDGFB orchestrates cellular proliferative responses and drives growth by virtue of its ability to activate PDGFRβ, which in turn triggers downstream signaling cascades facilitating DNA synthesis and regulating critical cell cycle checkpoints.
Customer Reviews (3)
Write a reviewThe seal of the reagent tubes is excellent, preventing evaporation and leakage.
With its unique design and construction, the experimental reagent provides me with a fresh experimental experience.
The stability of this reagent is exceptional, ensuring the reliability of the experiment.
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