"PDGFB" Related Products

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Recombinant Human PDGFB

Cat. No. : PDGFB-146H
Product Overview : Recombinant Human platelet-derived growth factor was expressed in modifiedhuman 293 cells.
Description : The platelet-derived growth factor (PDGF) family consists of four different polypeptide chains; PDGF-A, PDGF-B, PDGF-C and PDGF-D, sharing approximately 25% sequence homology. The biological effects of PDGF-B are exerted via two receptor tyrosine kinases, PDGFRA and PDGFRB. PDGF-B is expressed predominantly in platelets and the heart, but has been detected in the majority of human organs. PDGF-B is a potent mitogen for mesenchymal cells and plays a critical role in wound healing and tumor development. PDGF-B is an important growth factor contributing to angiogenesis and possibly lymphatic metastasis due to lymphangiogenesis. Additionally, PDGF-B plays a crucial role in wound healing. Studies have also suggested that PDGF-B is involved in the CNS including having a neuroprotective or neurotrophic effect in the developing or adult CNS, promoting angiogenic responses to injury in the CNS and remyelination..
Source : human 293 cells.
Molecular Mass : Under reducing conditions PDGF-B migrates as a broad band between 15.2 and 16.1 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified PDGF-B polypeptide that has a predicted monomeric molecular mass of 12.29 kDa.
PI : PDGF-B has a predicted pI of 9.38.
% Carbohydrate : purified PDGF-B consists of 30-45% carbohydrate by weight.
Glycosylation : PDGF-B contains N-linked oligosaccharides and a predicted O-linked Mannose.
Purity : >95%, as determined by SDS-PAGE, visualized by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C.
Activity : The ED50of PDGF-B is typically 14-25 ng/mL as measured in a cell proliferation assay using the murine Balb/3T3 fibroblast cell line.
Theoretical Sequence : SLGSLTI AEPAMIAECK TRTEVFEISRRLI DRTNANFLVWPPC VEVQRCSGCCNNR NV QCRP TQVQLRPV QVRKI EIVRK KPIFKK ATVTLEDHLACKCETVAAARPV.
PDB rendering based on 1pdg. :

Gene Name : PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ]
Synonyms : platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; platelet-derived growth factor beta; becaplermin; PDGF, B chain; platelet-derived growth factor 2; platelet-derived growth factor, B chain;Platelet-derived growth factor, beta polypeptide (oncogene SIS); oncogene SIS; Becaplermin; PDGF-2; PDGF subunit B; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor B chain
Gene ID : 5155
mRNA Refseq : NM_002608
Protein Refseq : NP_002599
MIM : 190040
UniProt ID : P01127
Chromosome Location : 22q13.1
Pathway : Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Hemostasis; Signaling by PDGF
Function : cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity

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