Active Glycosylated Recombinant Human PDGFB Homodimer

• Cat.No.: PDGFB-050H
• Product Overview: Platelet-derived growth factor (PDGF) is a homodimer composed of two glycosylated polypeptide chains. Each chain contains 109 amino acid residues and has a molecular weight of approximately 24.5 kDa. It is a glycosylated protein expressed in yeast.

Specification

• Species: Human
• Source: Yeast
• Description: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants.
• Form: Sterile lyophilized powder
• Molecular Mass: 12.1kDa (Monomer)
• Bio-activity: Activity is determined by a cell-proliferation assay using the Balb/c 3T3 cell line. Specific activity ≥2 × 10^6 IU/mg.
• Endotoxin: <1 EU/μg determined by LAL.
• Purity: ≥97%
• Storage: Lyophilized samples are stable for 36 months from the date of receipt when stored at –20 to –70 centigrade. After reconstitution, the cytokine can be kept for one month at 2–8 centigrade under sterile conditions or for three months at –20 to –70 centigrade in a manual-defrost freezer without significant loss of activity. Avoid repeated freeze–thaw cycles.
• Storage Buffer: This product is provided as a sterile lyophilized powder in a buffer system consisting of 100 mM glycine and 150 mM NaCl at pH 3.0.
• Reconstitution: Before opening the vial, centrifuge at 10, 000 rpm for 30–60 seconds, or at 3, 000 rpm for 5 minutes, then reconstitute with 0.2 mL of sterile water.

Gene Information

• Gene Name: PDGFB platelet derived growth factor subunit B [ Homo sapiens (human) ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet derived growth factor subunit B; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; platelet-derived growth factor subunit B; PDGF subunit B; PDGF, B chain; becaplermin; epididymis secretory sperm binding protein; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127