Recombinant E.coli Thiol: Disulfide Interchange Protein DsbC

• Cat.No.: DsbC-526E
• Product Overview: Recombinant DsbC was expressed inE.coliand purified by using conventional chromatography techniques. MW = 23.4kDa (216aa).

Specification

• Cat. No.: DsbC-526E
• Description: Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236 amino acids containing signal peptide (1-20 amino acids).
• Source: E.coli.
• Amino Acid Sequence: DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT DDGKHIIQGP MYDVSGTAPV NVTNKMLLKQ LNALEKEMIV YKAPQEKHVI TVFTDITCGY CHKLHEQMAD YNALGITVRY LAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YVLGVQLGVS GTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK.
• Form: Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 2 mM EDTA.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: dsbC thiol:disulfide interchange protein DsbC [ Escherichia coli APEC O1 ]
• Synonyms: DsbC; thiol:disulfide interchange protein DsbC
• Gene ID: 4494927
• mRNA Refseq: YP_854112

Reviews

02/08/2023

Timely support for protein expression optimization, appreciated assistance.

01/08/2023

High-quality protein characterization, fundamental for our scientific work.

12/15/2022

Accurate protein structural analysis, indispensable for our investigations.

Q&As

What is the primary biological function of DSBC protein?

The primary function of DSBC protein can be determined through literature review and experimental investigation.

What are the post-translational modifications (PTMs) that DSBC protein undergoes?

Detect post-translational modifications (PTMs) using mass spectrometry or Western blotting.

Can you recommend specific assays or techniques for quantifying the expression levels of DSBC protein in different tissues or under various experimental conditions?

Quantify DSBC protein expression with methods like quantitative PCR, Western blotting, or mass spectrometry under various conditions.

What cellular or physiological processes are regulated by DSBC protein?

Study DSBC protein's regulation of cellular processes through gene manipulation and biochemical assays.

What are the known molecular interactions or binding partners of DSBC protein?

Known molecular interactions or binding partners of DSBC protein can be identified using techniques like co-immunoprecipitation or mass spectrometry.

Can you suggest experimental techniques for characterizing the subcellular localization of DSBC protein within cells?

Techniques for subcellular localization include immunofluorescence staining, subcellular fractionation, and fluorescence microscopy.

Are there any known functional domains or motifs within DSBC protein?

Investigate functional domains within DSBC protein through mutagenesis and functional assays.