Cat. No.: |
PCNA-536H |
Product Overview: |
Recombinant human Proliferating Cell Nuclear Antigen, was expressed inE.coliand purified by conventional chromatography techniques. MW = 28 kDa (261 aa). |
Description: |
PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. |
Source: |
Escherichia Coli. |
Form: |
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 2 mM EDTA 20% glycerol. |
Purity: |
> 95% by SDS - PAGE. |
Concentration: |
1 mg/ml (determined by Bradford assay). |
Sequences Of Amino Acids: |
MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLR SEG FDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVF EAP NQE K VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLN FFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S. |
Storage: |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |