||Recombinant human Proliferating Cell Nuclear Antigen, was expressed inE.coliand purified by conventional chromatography techniques. MW = 28 kDa (261 aa).
||PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours.
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 2 mM EDTA 20% glycerol.
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLR SEG FDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVF EAP NQE K VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLN FFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.