Recombinant Human CSNK2A1, His-tagged CSNK2A1-199H

Recombinant Human CSNK2A1, His-tagged

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Recombinant Human CSNK2A1, His-tagged

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Cat. No. : CSNK2A1-199H
Product Overview : Recombinant human Protein Kinase Casein Kinase 2, fused to His tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 47.3 kDa (411 aa).
Description : Protein Kinase Casein Kinase 2 (PKCK2, also called CK2) is a ubiquitous Ser/Thr kinase expressed in all eukaryotes. PKCK2 is a tetramer composed of two catalytic kinase domains, alpha subunits, and two identical regulatory beta subunits. It has been implicated in cell cycle control , DNA repair, regulation of the circadian rhythm, and other cellular processes.
Source : Escherichia Coli.
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 500 mM NaCK, 50% glycerol.
Purity : > 90% by SDS - PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MSGPVPSRAR VYTDVNTHRP REYWDYESHV VE WGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKI LEN LRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LK AL DYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RV AS RY FKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG T E DLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LD KL LR YDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MM SG ISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1jwh. :

Gene Name : CSNK2A1 casein kinase 2, alpha 1 polypeptide [ Homo sapiens ]
Synonyms : CSNK2A1; casein kinase 2, alpha 1 polypeptide; CKII; CK2A1; casein kinase II alpha 1 subunit; protein kinase CK2; CK2 catalytic subunit alpha; EC 2.7.11.1; OTTHUMP00000029925; OTTHUMP00000029926
Gene ID : 1457
mRNA Refseq : NM_001895
Protein Refseq : NP_001886
MIM : 115440
UniProt ID : P68400
Chromosome Location : 20p13
Pathway : Adherens junction; Tight junction; Wnt signaling pathway
Function : ATP binding; nucleotide binding; protein N-terminus binding; protein serine/threonine kinase activity; transferase activity

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