||Recombinant human Protein Kinase Casein Kinase 2, fused to His tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 47.3 kDa (411 aa).
||Protein Kinase Casein Kinase 2 (PKCK2, also called CK2) is a ubiquitous Ser/Thr kinase expressed in all eukaryotes. PKCK2 is a tetramer composed of two catalytic kinase domains, alpha subunits, and two identical regulatory beta subunits. It has been implicated in cell cycle control , DNA repair, regulation of the circadian rhythm, and other cellular processes.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 500 mM NaCK, 50% glycerol.
||> 90% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MSGPVPSRAR VYTDVNTHRP REYWDYESHV VE WGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKI LEN LRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LK AL DYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RV AS RY FKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG T E DLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LD KL LR YDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MM SG ISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.