| Cat. No.: | HGFAC-144H |
| Product Overview: | Recombinant Human HGF activator encoding the complete human HGF protein isoform 1 (comprising the signal peptide, and alpha and beta chains) was expressed in modifiedhuman 293 cells. |
| Description: | Hepatocyte growth factor (HGF) consists of two subunits linked by a disulfide bond. The alpha subunit of HGF has a length of 440 amino acids and the beta subunit has a length of 234 amino acids. Both subunits of HGF are obtained by endoproteolytic cleavage of a common preproprotein which itself is biologically inactive. |
| Source: | Human 293 cells. |
| Amino Acid Sequence: | QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS. |
| Molecular Mass: | Under reducing conditions HGF migrates as a broad band between 80 and110 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. |
| pI: | HGF separates into a number of isoforms with a pI between 6 and 9 in 2D PAGE due to post-translational modifications, in particular glycosylation. |
| % Carbohydrate: | Purified HGF consists of 0-30% carbohydrate by weight. |
| Glycosylation: | HGF contains N- and O-linked oligosaccharides. |
| Purity: | >95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue. |
| Formulation: | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution: | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage: | Lyophilised products should be stored at 2 to 8°C. |
| Activity: | The ED50of HGF is typically 20 - 40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line. |
| Gene Name: | HGFAC HGF activator [ Homo sapiens ] |
| Synonyms: | HGFAC; HGF activator; HGFA; MGC138395; MGC138397; EC 3.4.21.;Hepatocyte growth factor activator; Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain |
| Gene ID: | 3083 |
| mRNA Refseq: | NM_001528 |
| Protein Refseq: | NP_001519 |
| UniProt ID: | Q04756 |
| Chromosome Location: | 4p16 |
| MIM: | 604552 |
| Function: | peptidase activity; protein binding; serine-type endopeptidase activity |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All HGFAC Products
Download Datasheet