Recombinant Human HGF Activator HGFAC-144H

Recombinant Human HGF Activator

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Recombinant Human HGF Activator

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Cat. No. : HGFAC-144H
Product Overview : Recombinant Human HGF activator encoding the complete human HGF protein isoform 1 (comprising the signal peptide, and alpha and beta chains) was expressed in modifiedhuman 293 cells.
Description : Hepatocyte growth factor (HGF) consists of two subunits linked by a disulfide bond. The alpha subunit of HGF has a length of 440 amino acids and the beta subunit has a length of 234 amino acids. Both subunits of HGF are obtained by endoproteolytic cleavage of a common preproprotein which itself is biologically inactive.
Source : Human 293 cells.
Amino Acid Sequence : QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS.
Molecular Mass : Under reducing conditions HGF migrates as a broad band between 80 and110 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation.
pI : HGF separates into a number of isoforms with a pI between 6 and 9 in 2D PAGE due to post-translational modifications, in particular glycosylation.
% Carbohydrate : Purified HGF consists of 0-30% carbohydrate by weight.
Glycosylation : HGF contains N- and O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilised products should be stored at 2 to 8°C.
Activity : The ED50of HGF is typically 20 - 40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line.
PDB rendering based on 1bht. :

Gene Name : HGFAC HGF activator [ Homo sapiens ]
Synonyms : HGFAC; HGF activator; HGFA; MGC138395; MGC138397; EC 3.4.21.;Hepatocyte growth factor activator; Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain
Gene ID : 3083
mRNA Refseq : NM_001528
Protein Refseq : NP_001519
UniProt ID : Q04756
Chromosome Location : 4p16
MIM : 604552
Function : peptidase activity; protein binding; serine-type endopeptidase activity

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