Active Recombinant Human CNTN5 protein, His-tagged

• Cat.No.: CNTN5-673H
• Product Overview: Active Recombinant Human CNTN5 protein(NP_055176.1) (Met 1-Gln 1059) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-1059 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CNTN5 coated plates (0.8 μg/ml and 100 μl/well), approximately 30%-50% will adhere specifically after 60 minutes at 37 °C.
• Molecular Mass: The secreted recombinant human CNTN5 comprises 1052 amino acids with a predicted molecular mass of 116 kDa. As a result of glycosylation, rhCNTN5 migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CNTN5 contactin 5 [ Homo sapiens ]
• Official Symbol: CNTN5
• Synonyms: CNTN5; contactin 5; contactin-5; hNB 2; NB 2; neural adhesion molecule; neural recognition molecule NB-2; NB-2; HNB-2s; MGC163491
• Gene ID: 53942
• mRNA Refseq: NM_001243270
• Protein Refseq: NP_001230199
• MIM: 607219
• UniProt ID: O94779