Active Recombinant Human IL13RA2 protein(Met1-Leu342), His&hFc-tagged

• Cat.No.: IL13RA2-765H
• Product Overview: Recombinant Human IL13Rα2 (NP_000631.1) (Met 1-Leu 342) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Leu342
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human IL13Ra2-Fch at 2 μg/mL (100 μl/well) can bind biotinylated human IL13, The EC50 of bind biotinylated human IL13 is 4-20 ng/mL.
• Molecular Mass: The recombinant human IL13Rα2/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 563 amino acids and predicts a molecular mass of 65 kDa. As a result of glycosylation, the rh IL13Rα2/Fc monomer migrates as an approximately 90-100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL13RA2 interleukin 13 receptor, alpha 2 [ Homo sapiens ]
• Official Symbol: IL13RA2
• Synonyms: IL13RA2; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; cancer/testis antigen 19; CD213a2; CT19; IL 13R; IL13BP; IL-13RA2; IL-13R-alpha-2; IL-13R subunit alpha-2; IL-13 receptor subunit alpha-2; interleukin 13 binding protein; interleukin-13-binding protein; interleukin 13 receptor alpha 2 chain; IL-13R; CD213A2
• Gene ID: 3598
• mRNA Refseq: NM_000640
• Protein Refseq: NP_000631
• MIM: 300130
• UniProt ID: Q14627