Active Recombinant Human KLK13 protein(Met1-Ile262), His-tagged

• Cat.No.: KLK13-302H
• Product Overview: Recombinant Human KLK13 (NP_056411.1) (Met 1-Ile 262) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ile262
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >200 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form)
• Molecular Mass: The recombinant human KLK13 consists of 256 amino acids and has a predicted molecular mass of 28.4 kDa. As a result of glycosylation, the apparent molecular mass of rh KLK13 is approximately 33-37 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLK13 kallikrein-related peptidase 13 [ Homo sapiens ]
• Official Symbol: KLK13
• Synonyms: KLK13; kallikrein-related peptidase 13; kallikrein 13; kallikrein-13; KLK L4; kallikrein-like gene 4; kallikrein-like protein 4; KLKL4; KLK-L4; DKFZp586J1923
• Gene ID: 26085
• mRNA Refseq: NM_015596
• Protein Refseq: NP_056411
• MIM: 605505
• UniProt ID: Q9UKR3