Recombinant Mouse Serpinf1 protein(Met1-Thr417), His-tagged

• Cat.No.: Serpinf1-858M
• Product Overview: Recombinant Mouse SerpinF1 (NP_035470.3)(Met 1-Thr 417) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Thr417
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized mouse SERPINF1-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CSNK2A1 with a linear range of 0.31-2.5 μg/ml.
• Molecular Mass: The secreted recombinant mouse SerpinF1consists of 409 amino acids and has a calculated molecular mass of 45.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Neurovascular protection by targeting early blood–brain barrier disruption with neurotrophic factors after ischemia–reperfusion in rats (2013)

Gene Information

• Gene Name: Serpinf1 serine (or cysteine) peptidase inhibitor, clade F, member 1 [ Mus musculus ]
• Official Symbol: Serpinf1
• Synonyms: SERPINF1; serine (or cysteine) peptidase inhibitor, clade F, member 1; pigment epithelium-derived factor; SDF-3; caspin; serpin F1; serpin-F1; alpha-2 antiplasmin; stromal cell derived factor 3; stromal cell-derived factor 3; pigment epithelium derived factor; serine (or cysteine) proteinase inhibitor, clade F, member 1; serine (or cysteine) proteinase inhibitor, clade F), member 1; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Pedf; Sdf3; EPC-1; Pedfl; AI195227
• Gene ID: 20317
• mRNA Refseq: NM_011340
• Protein Refseq: NP_035470