Active Recombinant Human APOA1 protein

• Cat.No.: APOA1-698H
• Product Overview: Recombinant Human APOA1 protein(Asp25-Gln267) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 25-267 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human A-I/APOA1 at 5μg/mL (100 μL/well) can bind Human MSR1/CD204 with a linear range of 1-49.4 ng/mL.
• Molecular Mass: 28.28 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
• Publications:
  • Imipramine and olanzapine block apoE4-catalyzed polymerization of Aβ and show evidence of improving Alzheimer’s disease cognition (2021)

Gene Information

• Gene Name: APOA1 apolipoprotein A-I [ Homo sapiens ]
• Official Symbol: APOA1
• Synonyms: APOA1; apolipoprotein A-I; apo-AI; apoA-I; MGC117399
• Gene ID: 335
• mRNA Refseq: NM_000039
• Protein Refseq: NP_000030
• MIM: 107680
• UniProt ID: P02647

Citation

Imipramine and olanzapine block apoE4-catalyzed polymerization of Aβ and show evidence of improving Alzheimer’s disease cognition

Journal: Alzheimer's Research & Therapy    PubMed ID: 35768831    Data: 2022/6/29

Authors: Noah R. Johnson, Athena C.-J. Wang, Huntington Potter

Article Snippet:Once the optimal concentrations of approximately 20 μM Aβ42, 1 nM apoE, and 15 μM ThT were determined, they were maintained throughout subsequent studies unless noted otherwise.Once the optimal concentrations of approximately 20 μM Aβ42, 1 nM apoE, and 15 μM ThT were determined, they were maintained throughout subsequent studies unless noted otherwise.. For HTS assay validation, recombinant human Aβ40 (rPeptide), recombinant human scrambled Aβ42 (rPeptide), recombinant human apoE2 and apoE3 (Creative BioMart), recombinant human apolipoprotein A-I (apoA-I; Creative BioMart), human plasma-derived apoE (Sigma), or dimethyl sulfoxide (DMSO; Sigma) were included or substituted at the indicated concentrations.. In assay optimization experiments, 3?8 wells were used per group and experiments were replicated one or two times, as indicated in the figure legends.In assay optimization experiments, 3?8 wells were used per group and experiments were replicated one or two times, as indicated in the figure legends.