||The CCAAT-box Binding Protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator. It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes. Cloning and functional characterization of the protein revealed that CTF1 contains a N-terminal DNA binding domain and a C-terminal proline-rich activation domain. CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes, with the forkhead thyroid transcription factor TTF2 to regulate hormonedependent transcription of thyroperoxidase gene, as well as other receptor proteins to synergize hormone receptor-responsive activation. The proline-rich activation domain of human CTF1 protein (amino acids 399-499) is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter. GST-CTF1 has been used for protein-protein interaction assays.
||For in vitro use only.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||100 ng are sufficient for a protein-protein interaction assay.
||> 95% by SDS-PAGE.
||Quality guaranteed for 12 months, Store at -80℃. Avoid freeze / thaw cycles.