||Recombinant Human IL1F10 expressed inE.coliwasencoding the human IL1F10 (Met1–Trp152) was recombined with a Hexa-histidine tag at the N-Terminus and purified by Ni-NTA column. MW=17.0 KD.
||IL1F10 binds soluble IL-1 receptor type 1. It was expressed in fetal skin, spleen and tonsil. It was expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.
||Human IL1F10 (Met1–Trp152)1 MCSLPMARYY IIKYADQKAL YTRDGQLLVG DPVADNCCAE KICTLPNRGL DRTKVPIFLG IQGGSRCLAC VETEEGPSLQ LEDVNIEELY KGGEEATRFT FFQSSSGSAF RLEAAAWPGW FLCGPAEPQQ PVQLTKESEP SARTKFYFEQ SW 152
||Lyophilized 100μg human IL1F10 in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free.
||>95% by SDS gel.
||Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL . Allow to set at least 30 minutes at 4℃, mix well.
||Store lyophilized protein at -20℃ or -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at -20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.