Recombinant Human Interleukin 1 Family, Member 10, His-tagged

• Cat.No.: IL1F10-157H
• Product Overview: Recombinant Human IL1F10 expressed inE.coliwasencoding the human IL1F10 (Met1–Trp152) was recombined with a Hexa-histidine tag at the N-Terminus and purified by Ni-NTA column. MW=17.0 KD.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-152 a.a.
• Description: IL1F10 binds soluble IL-1 receptor type 1. It was expressed in fetal skin, spleen and tonsil. It was expressed mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil.
• Sequence: Human IL1F10 (Met¹–Trp¹⁵²)1 MCSLPMARYY IIKYADQKAL YTRDGQLLVG DPVADNCCAE KICTLPNRGL DRTKVPIFLG IQGGSRCLAC VETEEGPSLQ LEDVNIEELY KGGEEATRFT FFQSSSGSAF RLEAAAWPGW FLCGPAEPQQ PVQLTKESEP SARTKFYFEQ SW 152
• Formulation: Lyophilized 100μg human IL1F10 in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free.
• Purity: >95% by SDS gel.
• Application: Cell biology.
• Reconstitution: Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL . Allow to set at least 30 minutes at 4℃, mix well.
• Storage: Store lyophilized protein at -20℃ or -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at -20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.

Gene Information

• Gene Name: IL1F10 interleukin 1 family, member 10 (theta) [ Homo sapiens ]
• Synonyms: IL1F10; interleukin 1 family, member 10 (theta); FKSG75; IL-1HY2; IL1-theta; MGC119831; MGC119832; MGC119833; FIL1-theta; interleukin-1 HY2; IL-1F10 (canonical form IL-1F10a); interleukin-1 receptor antagonist FKSG75; interleukin-1 receptor antagonist-like FIL1 theta; Interleukin-1 family member 10; IL-1F10; Interleukin-1 receptor antagonist-like FIL1 theta; Interleukin-1 theta; IL-1 theta; FIL1 theta; Interleukin-1 HY2; IL-1HY2
• Gene ID: 84639
• mRNA Refseq: NM_032556
• Protein Refseq: NP_115945
• MIM: 17144
• UniProt ID: Q8WWZ1
• Chromosome Location: 2q13
• Function: cytokine activity; interleukin-1 receptor antagonist activity

Citation

Pulmonary Delivery of Engineered Exosomes to Suppress Postoperative Melanoma Lung Metastasis through Preventing Premetastatic Niche Formation

Data: 2021/12/15

Authors: Xiaoqing Han, Luopeng Bi, Haiyuan Zhang

Article Snippet:Identification of GFE1 expression on exosomes.Identification of GFE1 expression on exosomes.. 10 μg of His-tagged recombinant DPEP1417 (Creative BioMart, USA) was incubated with 100 μg of BExoI or GExoI for 4 h at 4℃.. Then BExoI or418 GExoI was purified by ultracentrifugation.Then BExoI or418 GExoI was purified by ultracentrifugation.