Active Recombinant Human General Transcription Factor IIA, 1, 19/37kDa
Cat.No. : | GTF2A1-1170H |
- Specification
- Gene Information
- Related Products
Cat. No. : | GTF2A1-1170H |
Description : | The Transcription Factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α-subunit), 19 kDa (β-subunit) and 12 kDa (γ-subunit). The α- and β-subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ-subunit), retains native TFIIA activity. The p55 subunit of TFIIA is isolated from a strain of E. coli that contains the coding sequence of human p55 of TFIIA under the control of T7 promoter. The p12 subunit of TFIIA is isolated from a strain ofE. colithat contains the coding sequence for human TFIIA γ-subunit under the control of T7 promoter. Recombinant TFIIA can be used to super-shift TBPDNA complex in a gel mobility shift assay and to stimulate activator-dependent transcriptionin vitro. TFIIA is greater than 95% pure and does not contain any detectable protease, DNase, or RNase activities. |
Source : | E. coli. |
Usage : | For in vitro use only. |
Form : | Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol. |
Activity : | 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction, 20 ng are sufficient for in vitro transcription assay and 100 ng are sufficient for protein-protein interaction assays. |
Purity : | > 95% by SDS-PAGE. |
Storage : | Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles. |
Gene Name : | GTF2A1 general transcription factor IIA, 1, 19/37kDa [ Homo sapiens ] |
Synonyms : | GTF2A1; general transcription factor IIA, 1, 19/37kDa; TF2A1; TFIIA; MGC129969; MGC129970; TFIIA alpha, p55, isoform 1; glucose regulated protein, 58kD pseudogene; TFIIA-42;Transcription initiation factor IIA subunit 1; Transcription initiation factor IIA alpha chain; TFIIA p35 subunit; TFIIA p19 subunit |
Gene ID : | 2957 |
mRNA Refseq : | NM_015859 |
Protein Refseq : | NP_056943 |
MIM : | 600520 |
UniProt ID : | P52655 |
Chromosome Location : | 14q31 |
Pathway : | Basal transcription factors; Gene Expression; HIV Infection; Transcription |
Function : | DNA binding; TATA-binding protein binding; general RNA polymerase II transcription factor activity; protein heterodimerization activity; transcription coactivator activity; transcription factor binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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