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Recombinant Human RXRA, Ligand Binding Domain

Cat. No. : RXRA-1151H
Description : Recombinant His tagged RXR-LBD is isolated from an E.coli strain that carries the coding sequence of the human RXR-alpha ligand binding domain under the control of a T7 promoter. Retinoid X receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain (LBD). RXR partners include thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, several constitutive active orphan nuclear receptors (e.g. Nuclear Growth Factor I-B), oxysterol receptors, and constitutive androstane receptors. RXRs also form homodimers to mediate the effects of 9-cisretinoic acid (9-cRA). Depending on these protein- protein interactions, RXR-containing complexes have distinct ligand-dependent and constitutive functions. The LBD is functionally complex and mediates ligand binding, receptor homo- and heterodimerization, repression of transcription in the absence of ligand, and ligand-dependent activation of transcription. Hormone binding to the structurally conserved LBD of the RXR triggers a conformational change that principally affects the conserved C-terminal transactivation helix H12 involved in transcriptional activation. Recombinant RXR-LBD is isolated from an E.coli strain that carries the coding sequence of the human RXRLBD under the control of a T7 promoter.
Source : E.coli.
Form : Liquid. Supplied in 20mM Tris-HCl, pH8.0, 100mM KCl, 0.2mM EDTA, 1mM DTT, 20% glycerol.
Activity : 20ng are sufficient for an in vitro transcription assay and 100ng are sufficient for a protein-protein interaction assay.
Application : RXR-LBD has been applied in in vitro transcription assays, DNA, and protein-protein interactions assays.
Purity : >95% by SDS-PAGE.
Usage : For in vitro use only.
Storage : Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.
PDB rendering based on 1by4. :

Gene Name : RXRA retinoid X receptor, alpha [ Homo sapiens ]
Synonyms : RXRA; FLJ00280; FLJ00318; FLJ16020; FLJ16733; MGC102720; NR2B1; OTTHUMP00000022510; Retinoid X receptor alpha; retinoid X receptor, alpha; Nuclear receptor subfamily 2 group B member 1; Retinoid X receptor, alpha; Retinoid X receptor alpha (RXR-alpha).
Gene ID : 6256
mRNA Refseq : NM_002957
Protein Refseq : NP_002948
MIM : 180245
UniProt ID : P19793
Chromosome Location : 9q34.3
Pathway : Adipocytokine signaling pathway; Non-small cell lung cancer; PPAR signaling pathway; Small cell lung cancer; Thyroid cancer.
Function : ligand-regulated transcription factor activity; metal ion binding; protein binding; retinoid-X receptor activity; sequence-specific DNA binding; steroid binding; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity; vitamin D receptor binding; vitamin D response element binding; zinc ion binding.

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