Recombinant Human Rho GDP Dissociation Inhibitor (GDI) Beta

• Cat.No.: ARHGDIB-1277H
• Product Overview: Recombinant Human D4-GDI full length protein expressed inE.colishows a 54 kDa SDS-PAGE. The D4-GDI is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: ARHGDIB-1277H
• Description: D4-GDI belongs to the family of Rho-GDIs or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI-1, D4 GDI/RhoGDI-2 and RhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and is involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. Cells deficient of D4-GDI revert to a normal breast epithelial phenotype characterized by the formation of cysts with a central lumen when grown on Matrigel. D4-GDI reduction is linked to a down-regulation of the cell surface protein ß1-integrin and a loss of cell-matrix adhesion, suggestive of D4-GDI induction of cell invasion by controlling ß1-integrin expression. The expression of ß1-integrin and invasive activities are restored once there's restitution of D4-GDI expression to the levels found in the parental MDA-MB-231 cells. Therefore it is suggested that D4-GDI may play a significant role in the regulation of breast cancer cell invasiveness.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered clear solution.
• Formulation: D4-GDI protein at 0.1mg/ml in 50mM Tris-acetate, pH7.5, 20% glycerol and 1mM EDTA.
• Stability: Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: ELISA, Inhibition Assays, Western Blotting.

Gene Information

• Gene Name: ARHGDIB Rho GDP dissociation inhibitor (GDI) beta [ Homo sapiens ]
• Synonyms: ARHGDIB; Rho GDP dissociation inhibitor (GDI) beta; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2; Rho GDI 2; Rho GDP-dissociation inhibitor 2
• Gene ID: 397
• mRNA Refseq: NM_001175
• Protein Refseq: NP_001166
• MIM: 602843
• UniProt ID: P52566
• Chromosome Location: 12p12.3
• Pathway: Neurotrophin signaling pathway; Signaling by Rho GTPases
• Function: GTPase activator activity; Rho GDP-dissociation inhibitor activity; protein binding