Active Recombinant Staphylococcal Spa

• Cat.No.: spa-1286S
• Product Overview: Recombinant Staphylococcal Protein A produced inE.Coliwas a non-glycosylated, Polypeptide chain having a molecular mass of 45 kDa. Recombinant Staphylococcal Protein A is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: spa-1286S
• Description: Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered clear colorless solution.
• Formulation: The protein solution contains no additives.
• Activity: Greater than 95.0% binding activity to human IgG.
• Purity: Greater than 98.0% as determined by RP-HPLC.
• Stability: SPA should be stored at -20°C.

Gene Information

• Gene Name: spa immunoglobulin G binding protein A precursor (protein A) [ Staphylococcus aureus subsp. aureus str. Newman ]
• Synonyms: spa; immunoglobulin G binding protein A precursor (protein A)
• Gene ID: 5332074
• Protein Refseq: YP_001331090

Reviews

12/13/2022

The SPA protein's superior performance in ELISA paves the way for robust and precise data analysis, providing invaluable insights into various biological processes.

07/26/2017

I am genuinely excited to embark on this scientific journey, empowered by the superior SPA protein and the manufacturer's unwavering commitment to ensuring my success.

06/20/2017

The SPA protein comes highly recommended due to its exceptional performance in ELISA assays.

Q&As

Can SPA protein be used in the purification of recombinant proteins?

Yes, SPA protein is effective in purifying recombinant proteins, aiding in the production of high-quality proteins for research and therapeutic applications.

Can SPA protein be employed in cancer research?

Yes, SPA protein is valuable in cancer research, assisting in the identification and analysis of cancer-specific antibodies that may be potential targets for treatment.

Can SPA protein be utilized in infectious disease diagnosis?

Yes, SPA protein is applied in infectious disease diagnosis to detect antibodies produced in response to pathogens, aiding in the identification of the infecting agent.

How does SPA protein enhance the accuracy of Western blotting?

In Western blotting, SPA protein is used to bind and detect specific antibodies, improving the accuracy of protein identification and quantification in clinical samples.

How does SPA protein contribute to therapeutic antibody production?

SPA protein is utilized in the purification process of therapeutic antibodies, helping to isolate and obtain high-purity antibodies for use in medical treatments.