||Recombinant Human AHSP protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 11.8 kDa (102aa).
||AHSP(Alpha-hemoglobin stabilizing protein), also known as ERAF(Erythroid associated factor), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of α-hemoglobin during normal erythroid cell development. It specifically protects free α-hemoglobin from precipitation in live cells and in solution. This protein is downregulated in transmissible spongiform encephalopathies (TSEs). It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.
|Amino Acid Sequence:
||MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||< 1.0 EU per 1 μg of protein (determined by LAL method).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.