||Recombinant human PEBP1 was expressed inE.coliand purified by using conventional chromatography techniques. MW =21 kDa (187aa).
||Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.
|Amino Acid Sequence:
||MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
||> 95% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||< 1.0 EU per 1 μg of protein (determined by LAL method).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.