Recombinant Human Glycogen Phosphorylase B PYGB-366H

Recombinant Human Glycogen Phosphorylase B

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Recombinant Human Glycogen Phosphorylase B

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Cat. No. : PYGB-366H
Product Overview : Recombinant Human Glycogen Phosphorylase produced inE.Coliis a single, non-glycosylated, polypeptide chain. The Human GPBB mature chain: 2 - 843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40. The GPBB is purified by proprietary chromatographic techniques.
Description : Glycogen phosphorylase is one of the phosphorylaseenzymes (EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase. Glycogen phosphorylase can only act on linearchainsof glycogen (a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch (which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered colourless liquid formualtion.
Formulation : 0.8 mg/1ml, each mg of protein contains 50% glycerol.
Applications : Immunoassays and western blot.
Purity : Greater than 85.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Storage : GPBB although stable at 10°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Gene Name : PYGB phosphorylase, glycogen; brain [ Homo sapiens ]
Synonyms : PYGB; phosphorylase, glycogen; brain; MGC9213; glycogen phosphorylase B; EC 2.4.1.1; GPBB
Gene ID : 5834
mRNA Refseq : NM_002862
Protein Refseq : NP_002853
MIM : 138550
UniProt ID : P11216
Chromosome Location : 20p11.2-p11.1
Pathway : Insulin signaling pathway; Starch and sucrose metabolism
Function : glycogen phosphorylase activity; pyridoxal phosphate binding; transferase activity, transferring glycosyl groups

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