Recombinant Human PYGB protein, His & T7-tagged

• Cat.No.: PYGB-7817H
• Product Overview: Recombinant Human PYGB aa. (Pro477~Glu731 (Accession # P11216)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.3kDa
• Protein length: Pro477~Glu731 (Accession # P11216)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PYGB glycogen phosphorylase B [ Homo sapiens (human) ]
• Official Symbol: PYGB
• Synonyms: PYGB; glycogen phosphorylase B; GPBB; glycogen phosphorylase, brain form; phosphorylase, glycogen; brain
• Gene ID: 5834
• mRNA Refseq: NM_002862.3
• Protein Refseq: NP_002853.2
• UniProt ID: P11216