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Recombinant Human Glycogen Phosphorylase B

Cat. No.: PYGB-366H
Product Overview: Recombinant Human Glycogen Phosphorylase produced inE.Coliis a single, non-glycosylated, polypeptide chain. The Human GPBB mature chain: 2 - 843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40. The GPBB is purified by proprietary chromatographic techniques.
Description: Glycogen phosphorylase is one of the phosphorylaseenzymes (EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase. Glycogen phosphorylase can only act on linearchainsof glycogen (a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch (which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered colourless liquid formualtion.
Formulation: 0.8 mg/1ml, each mg of protein contains 50% glycerol.
Applications: Immunoassays and western blot.
Purity: Greater than 85.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Storage: GPBB although stable at 10°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Gene Name: PYGB phosphorylase, glycogen; brain [ Homo sapiens ]
Synonyms: PYGB; phosphorylase, glycogen; brain; MGC9213; glycogen phosphorylase B; EC 2.4.1.1; GPBB
Gene ID: 5834
mRNA Refseq: NM_002862
Protein Refseq: NP_002853
MIM: 138550
UniProt ID: P11216
Chromosome Location: 20p11.2-p11.1
Pathway: Insulin signaling pathway; Starch and sucrose metabolism
Function: glycogen phosphorylase activity; pyridoxal phosphate binding; transferase activity, transferring glycosyl groups

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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