||Recombinant Human Glycogen Phosphorylase produced inE.Coliis a single, non-glycosylated, polypeptide chain. The Human GPBB mature chain: 2 - 843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40. The GPBB is purified by proprietary chromatographic techniques.
||Glycogen phosphorylase is one of the phosphorylaseenzymes (EC220.127.116.11). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase. Glycogen phosphorylase can only act on linearchainsof glycogen (a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch (which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.
||Sterile Filtered colourless liquid formualtion.
||0.8 mg/1ml, each mg of protein contains 50% glycerol.
||Immunoassays and western blot.
||Greater than 85.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||GPBB although stable at 10°C for 7 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.