Recombinant Human DNA-damage-inducible Transcript 3, GST-tagged

• Cat.No.: DDIT3-1361H
• Product Overview: Recombinant Human DDIT3 full length protein fused to GST tag expressed inE.coli, shows a 52 kDa on SDS-PAGE. The DDIT3 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: DDIT3-1361H
• Description: GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. GADD153 expression is induced by a variety of cellular stresses, including glucose deprivation, exposure to genotoxic agents, UV irradiation, the acute phase reaction, and other growth-arresting situations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. In growing cells gadd153 is expressed at very low levels. Cell cycle arrest might be induced by forced expression of gadd153 in numerous types of cells; it might also induce cell death by apoptosis. Myc protein strongly stimulates cellular proliferation by inducing cells to exit G0/G1 and enter the cell cycle. Myc protein represses the expression of gadd153.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered clear solution.
• Formulation: DDIT3 protein at a concentration 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Applications: • ELISA • Western Blotting.
• Stability: Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: DDIT3 DNA-damage-inducible transcript 3 [ Homo sapiens ]
• Synonyms: DDIT3;DNA-damage-inducible transcript 3; CHOP; CEBPZ; CHOP10; GADD153; MGC4154; C/EBP zeta; C/EBP homologous protein; growth arrest- and DNA damage-inducible; CCAAT/enhancer-binding protein homologous protein;DNA damage-inducible transcript 3; DDIT-3; wth arrest and DNA-damage-inducible protein GADD153
• Gene ID: 1649
• mRNA Refseq: NM_004083
• Protein Refseq: NP_004074
• MIM: 126337
• UniProt ID: P35638
• Chromosome Location: 12q13.1-q13.2
• Pathway: MAPK signaling pathway; Diabetes pathways
• Function: protein dimerization activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity