||Recombinant murine NGAL (p25, SV-40-induced 24P3 protein, Lipocalin-2) is with a N-terminal GFP tag (Green fluorescent protein from Aequorea victoria). The expressed gene has been obtained merging the DNA codifying the GFP mutant at the N-term and the DNA of murine NGAL at C-term. The two proteins are connected by the linker GSAGSAAGAGAF. It is expressed inE. coli.
||Lipocalin-2 also called Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including α1-microglobulin and β-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke.
||> 95% by SDS-PAGE. Molecular weight > 45 kDa.
||Highly fluorescent GFP mutant (S65G, S72A) is a emits green light (emission maximum at 511 nm) when excited at 488 nm.
||NGAL GFP tagged Green Fluorescent Protein (GFP) is widely used as a fluorescent indicator for monitoring gene expression and protein ocalization in vivo.
||-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20oC. Avoid repeated freeze/defrost cycles.