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Recombinant Murine Lipocalin 2, GFP-tagged

Cat. No.: Lcn2-2739M
Product Overview: Recombinant murine NGAL (p25, SV-40-induced 24P3 protein, Lipocalin-2) is with a N-terminal GFP tag (Green fluorescent protein from Aequorea victoria). The expressed gene has been obtained merging the DNA codifying the GFP mutant at the N-term and the DNA of murine NGAL at C-term. The two proteins are connected by the linker GSAGSAAGAGAF. It is expressed inE. coli.
Description: Lipocalin-2 also called Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including α1-microglobulin and β-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke.
Source: E. coli.
Purity: > 95% by SDS-PAGE. Molecular weight > 45 kDa.
Activity: Highly fluorescent GFP mutant (S65G, S72A) is a emits green light (emission maximum at 511 nm) when excited at 488 nm.
Usage: NGAL GFP tagged Green Fluorescent Protein (GFP) is widely used as a fluorescent indicator for monitoring gene expression and protein ocalization in vivo.
Storage: -20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20oC. Avoid repeated freeze/defrost cycles.
Gene Name: Lcn2 lipocalin 2 [ Mus musculus ]
Synonyms:
Lcn2; lipocalin 2; 24p3; Sip24; AW212229; OTTMUSP00000013951; secreted inducible protein 24; NGAL
Gene ID: 16819
mRNA Refseq: 16819
Protein Refseq: NP_032517
UniProt ID: P11672
Chromosome Location: 2 27.0 cM
Function: binding; transporter activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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