Recombinant Human BRCA1 protein, his-tagged

• Cat.No.: BRCA1-27670TH
• Product Overview: Recombinant Human BRCA1 fused with his tag was expressed in Baculovirus-insect cell.

Specification

• Description: BRCA1 and BRCA1 are a human gene and its protein product, respectively. The official symbol (BRCA1, italic for the gene, nonitalic for the protein) and the official name (breast cancer 1) are maintained by the HGNC. Orthologs, styled Brca1 and Brca1, are common in other mammal species. BRCA1 is a human tumor suppressor gene (to be specific, a caretaker gene), found in all humans; its protein, also called by the synonym breast cancer type 1 susceptibility protein, is responsible for repairing DNA.
• Species: Human
• Tag: His
• Form: This protein was kept in 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.
• Molecular Mass: 209.1 kDa
• AA Sequence: MDLSALRVEEVQNVINAMQKILECP ICLELIKEPVSTKCDHIFCKFCMLK LLNQKKGPSQCPLCKNDITKRSLQE STRFSQLVEELLKIICAFQLDTGLE YANSYNFAKKENNSPEHLKDEVSII QSMGYRNRAKRLLQSEPENPSLQET SLSVQLSNLGTVRTLRTKQRIQPQK TSVYIELGSDSSEDTVNKATYCSVG DQELLQITPQGTRDEISLDSAKKAA CEFSETDVTNTEHHQPSNNDLNTTE KRAAERHPEKYQGSSVSNLHVEPCG TNTHASSLQHENSSLLLTKDRMNVE KAEFCNKSKQPGLARSQHNRWAGSK ETCNDRRTPSTEKKVDLNADPLCER KEWNKQKLPCSENPRDTEDVPWITL NSSIQKVNEWFSRSDELLGSDDSHD GESESNAKVADVLDVLNEVDEYSGS SEKIDLLASDPHEALICKSERVHSK SVESNIEDKIFGKTYRKKASLPNLS HVTENLIIGAFVTEPQIIQERPLTN KLKRKRRPTSGLHPEDFIKKADLAV QKTPEMINQGTNQTEQNGQVMNITN SGHENKTKGDSIQNEKNPNPIESLE KESAFKTKAEPISSSISNMELELNI HNSKAPKKNRLRRKSSTRHIHALEL VVSRNLSPPNCTELQIDSCSSSEEI KKKKYNQMPVRHSRNLQLMEGKEPA TGAKKSNKPNEQTSKRHDSDTFPEL KLTNAPGSFTKCSNTSELKEFVNPS LPREEKEEKLETVKVSNNAEDPKDL MLSGERVLQTERSVESSSISLVPGT DYGTQESISLLEVSTLGKAKTEPNK CVSQCAAFENPKGLIHGCSKDNRND TEGFKYPLGHEVNHSRETSIEMEES ELDAQYLQNTFKVSKRQSFAPFSNP GNAEEECATFSAHSGSLKKQSPKVT FECEQKEENQGKNESNIKPVQTVNI TAGFPVVGQKDKPVDNAKCSIKGGS RFCLSSQFRGNETGLITPNKHGLLQ NPYRIPPLFPIKSFVKTKCKKNLLE ENFEEHSMSPEREMGNENIPSTVST ISRNNIRENVFKEASSSNINEVGSS TNEVGSSINEIGSSDENIQAELGRN RGPKLNAMLRLGVLQPEVYKQSLPG SNCKHPEIKKQEYEEVVQTVNTDFS PYLISDNLEQPMGSSHASQVCSETP DDLLDDGEIKEDTSFAENDIKESSA VFSKSVQKGELSRSPSPFTHTHLAQ GYRRGAKKLESSEENLSSEDEELPC FQHLLFGKVNNIPSQSTRHSTVATE CLSKNTEENLLSLKNSLNDCSNQVI LAKASQEHHLSEETKCSASLFSSQC SELEDLTANTNTQDPFLIGSSKQMR HQSESQGVGLSDKELVSDDEERGTG LEENNQEEQSMDSNLGEAASGCESE TSVSEDCSGLSSQSDILTTQQRDTM QHNLIKLQQEMAELEAVLEQHGSQP SNSYPSIISDSSALEDLRNPEQSTS EKAVLTSQKSSEYPISQNPEGLSAD KFEVSADSSTSKNKEPGVERSSPSK CPSLDDRWYMHSCSGSLQNRNYPSQ EELIKVVDVEEQQLEESGPHDLTET SYLPRQDLEGTPYLESGISLFSDDP ESDPSEDRAPESARVGNIPSSTSAL KVPQLKVAESAQSPAAAHTTDTAGY NAMEESVSREKPELTASTERVNKRM SMVVSGLTPEEFMLVYKFARKHHIT LTNLITEETTHVVMKTDAEFVCERT LKYFLGIAGGKWVVSYFWVTQSIKE RKMLNEHDFEVRGDVVNGRNHQGPK RARESQDRKIFRGLEICCYGPFTNM PTDQLEWMVQLCGASVVKELSSFTL GTGVHPIVVVQPDAWTEDNGFHAIG QMCEAPVVTREWVLDSVALYQCQEL DTYLIPQIPHSHY
• Purity: 0.9
• Applications: 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay. Concentration is variable in different lots.
• Storage: Always store at -80 centigrade. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: BRCA1 BRCA1, DNA repair associated [ Homo sapiens (human) ]
• Official Symbol: BRCA1
• Synonyms: IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
• Gene ID: 672
• mRNA Refseq: NM_007294
• Protein Refseq: AAC37594
• MIM: 113705
• UniProt ID: P38398
• Chromosome Location: 17q21.31
• Pathway: ATF-2 transcription factor network, organism-specific biosystem (from Pathway Interaction Database)
  Androgen receptor signaling pathway, organism-specific biosystem (from WikiPathways)
  Aurora A signaling, organism-specific biosystem (from Pathway Interaction Database)
  BARD1 signaling events, organism-specific biosystem (from Pathway Interaction Database)