||Native Human Calpain-1 was purified from Human Erythrocyte.
||The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
||Frozen in 20 mM imidazole, 5mM β-mercaptoethanol, 1 mM EDTA, 1mM EGTA, 30% glycerol, pH 6.8
||Greater than or equal to 1,000 units per mg protein. One unit is de ned as the amount of enzyme that hydrolyzes one ρmole Suc-LLVY-AMC per minute at 25o C.
||Greater than or equal to 95% by SDS-PAGE.
||-70°C or lower