Active Native Human Calpain-1

• Cat.No.: CAPN1-27397TH
• Product Overview: Native Human Calpain-1 was purified from Human Erythrocyte.

Specification

• Description: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
• Source: Human Erythrocyte
• Species: Human
• Form: Frozen in 20 mM imidazole, 5mM β-mercaptoethanol, 1 mM EDTA, 1mM EGTA, 30% glycerol, pH 6.8
• Bio-activity: Greater than or equal to 1,000 units per mg protein. One unit is de ned as the amount of enzyme that hydrolyzes one ρmole Suc-LLVY-AMC per minute at 25o C.
• Molecular Mass: 110,000 Daltons
• Purity: Greater than or equal to 95% by SDS-PAGE.
• Storage: -70°C or lower

Gene Information

• Gene Name: CAPN1 calpain 1, (mu/I) large subunit [ Homo sapiens (human) ]
• Official Symbol: CAPN1
• Synonyms: CAPN1; calpain 1, (mu/I) large subunit; calpain-1 catalytic subunit; CANP; CANPL1; muCANP; muCL; CANP 1; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calpain, large polypeptide L1; calcium-activated neutral proteinase 1; cell proliferation-inducing protein 30; cell proliferation-inducing gene 30 protein
• Gene ID: 823
• mRNA Refseq: NM_001198868
• Protein Refseq: NP_001185797
• MIM: 114220
• UniProt ID: P07384
• Chromosome Location: 11q13
• Pathway: Alzheimers disease; Apoptosis; Focal Adhesion
• Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; protein binding