Recombinant Human Human CDK1

• Cat.No.: CCNB1-31593TH
• Product Overview: Recombinant full length Human CDK1 and CCNB1 with N terminal proprietary tags on both proteins; MWt 58 and 75kDa respectively.

Specification

• Description: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
• Source: Baculovirus infected sf9 cells
• Tissue specificity: Isoform 2 is found in breast cancer tissues.
• Biological activity: The Specific activity of CCNB1-31593TH was determined to be 14 nmol/min/mg.
• Form: Liquid
• Purity: Densitometry
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: CCNB1 cyclin B1 [ Homo sapiens ]
• Official Symbol: CCNB1
• Synonyms: CCNB1; cyclin B1; CCNB; G2/mitotic-specific cyclin-B1; G2/mitotic specific cyclin B1
• Gene ID: 891
• mRNA Refseq: NM_031966
• Protein Refseq: NP_114172
• MIM: 123836
• Uniprot ID: P14635
• Chromosome Location: 5q12
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem
• Function: histone kinase activity; kinase activity; protein binding; protein kinase binding