Recombinant Human CDK7, His-tagged

• Cat.No.: CCNH-31758TH
• Product Overview: Reconmbinant Full length human CDK7, Cyclin H1 and MNATI co-expressed by Baculovirus in Sf9 cells using an Nterminal His tag.

Specification

• Description: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
• Conjugation: HIS
• Biological activity: Specific activity: 19 nmol/min/mg.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: 150mM ImidazoleConstituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: CCNH cyclin H [ Homo sapiens ]
• Official Symbol: CCNH
• Synonyms: CCNH; cyclin H; cyclin-H; CAK complex subunit; CDK activating kinase complex subunit; cyclin dependent kinase activating kinase complex subunit; MO15 associated protein; p34; p37
• Gene ID: 902
• mRNA Refseq: NM_001199189
• Protein Refseq: NP_001186118
• MIM: 601953
• Uniprot ID: P51946
• Chromosome Location: 5q13.3-q14
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: contributes_to DNA-dependent ATPase activity; contributes_to RNA polymerase II carboxy-terminal domain kinase activity; protein binding; contributes_to protein kinase activity; protein kinase binding