Recombinant Human CDC25A, His-tagged

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Recombinant Human CDC25A, His-tagged

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Cat.No. : CDC25A-27899TH
Product Overview : Recombinant full length Human Cdc25A with N terminal His and DDDDK tags; predicted MWt 63.2kDa, 560 amino acids inclusive of tags;
Description : CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Protein length : 524 amino acids
Conjugation : HIS
Molecular Weight : 63.200kDa inclusive of tags
Source : E. coli
Form : Liquid
Purity : by SDS-PAGE
Storage buffer : Preservative: NoneConstituents: 20% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, 1mM EDTA, pH 8
Storage : Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Sequences of amino acids : MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMELG PEPPHRRRLL FACSPPPASQ PVVKALFGAS AAGGLSPVTN LTVTMDQLQGLGSDYEQPLE VKNNSNLQRM GSSESTDSGF CLDSPGPLDS KENLENPMRR IHSLPQKLLG CSPALKRSHS DSLDHDIFQL IDPDENKENEAFEFKKPVRP VSRGCLHSHG LQEGKDLFTQ RQNSAPARML SSNERDSSEP GNFIPLFTPQ SPVTATLSDE DDGFVDLLDG ENLKNEEETPSCMASLWTAP LVMRTTNLDN RCKLFDSPSL CSSSTRSVLK RPERSQEESP PGSTKRRKSM SGASPKESTN PEKAHETLHQ SLSLASSPKGTIENILDNDP RDLIGDFSKG YLFHTVAGKH QDLKYISPEI MASVLNGKFA NLIKEFVIID CRYPYEYEGG HIKGAVNLHM EEEVEDFLLKKPIVPTDGKR VIVVFHCEFS SERGPRMCRY VRERDRLGNE YPKLHYPELY VLKGGYKEFF MKCQSYCEPP SYRPMHHEDF KEDLKKFRTKSRTWAGEKSK REMYSRLKKL
Sequence Similarities : Belongs to the MPI phosphatase family.Contains 1 rhodanese domain.
Gene Name : CDC25A cell division cycle 25 homolog A (S. pombe) [ Homo sapiens ]
Official Symbol : CDC25A
Synonyms : CDC25A; cell division cycle 25 homolog A (S. pombe); cell division cycle 25 homolog A (S. cerevisiae) , cell division cycle 25A; M-phase inducer phosphatase 1;
Gene ID : 993
mRNA Refseq : NM_001789
Protein Refseq : NP_001780
MIM : 116947
Uniprot ID : P30304
Chromosome Location : 3p21
Pathway : Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem;
Function : hydrolase activity; protein binding; protein tyrosine phosphatase activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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